Interaction between a fluoroquinolone derivative and RNAs with a single bulge

• Published in: Journal of biochemistry (Tokyo) Vol. 171; no. 2; pp. 239 - 244
• Main Authors: Nagano, Konami, Kamimura, Takashi, Kawai, Gota
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 21-02-2022
• Subjects: Fluoroquinolones; Nucleic Acid Conformation; RNA - chemistry; RNA - genetics; RNA, Messenger; interaction; small molecule; NMR; RNA; fluoroquinolone
• ISSN: 0021-924X; 1756-2651
• DOI: 10.1093/jb/mvab124

Abstract
Interaction analysis between small molecules and RNA as well as structure determination of RNA–small molecule complexes will be the clues to search for compounds that bind to specific mRNA or non-coding RNA in drug discovery. In this study, the RNA-binding ability of a fluoroquinolone derivative, KG022, was examined against single-residue bulge-containing hairpin RNAs as RNA models. Nuclear magnetic resonance analysis indicated that KG022 interacts with the RNAs in the vicinity of the bulge residue, with preferring C and G as the bulge residues. The solution structures of the RNA–KG022 complexes showed that the KG022 binds to the RNAs at the bulge-out regions. Each substituent in KG022 interacts with specific position of RNAs around the bulge-out region probably contributing the specificity of the binding. This work provides a novel member for the RNA-targeted small molecules.
Graphical Abstract
Graphical Abstract