Polymorphism of exon 2-3 of bovine major histocompatibility complex class I BoLa-A gene

• Published in: Genetics and molecular biology Vol. 30; no. 3; pp. 560 - 566
• Main Authors: Bhushan, Bharat, Patra, Biswa Nath, Das, Pranab Jyoti, Dutt, Triveni, Kumar, Pushpendra, Sharma, Arjava, Umang, Dandapat, Satyabarta, Ahlawat, Shiv Pal Singh
• Format: Journal Article
• Language: English
• Published: Sociedade Brasileira de Genética 2007
• Subjects: BIOCHEMISTRY & MOLECULAR BIOLOGY; BoLa-A polymorphism; cattle; GENETICS & HEREDITY; MHC; BoLa-A polymorphism; MHC; cattle
• ISSN: 1415-4757; 1678-4685; 1415-4757; 1678-4685
• DOI: 10.1590/S1415-47572007000400009

The exon 2-3 region of bovine major histocompatibility complex (MHC) class I BoLa-A gene was investigated for polymorphisms in three breeds of cattle originated in the Indian subcontinent namely Sahiwal, Tharparkar, Hariana, as well as crossbred (Bos taurus x Bos indicus) cattle and Jersey, the exotic breed (Bos taurus). The PCR amplified fragment of 714 bp showed distinct DdeI-, TaqI- and HinfI- RFLP patterns, thus confirming a higher degree of polymorphism in this region. To our knowledge this is the first report of HinfI restriction patterns for BoLa-A exon 2-3. The sequencing results revealed a number of nucleotide substitutions in this region, which resulted in amino acid changes. The present investigation confirmed that MHC class I BoLa-A exon 2-3 is highly polymorphic in cattle.