In Vitro Anti-Inflammatory and Immunomodulatory Activities of an Extract from the Roots of Bupleurum rotundifolium

• Published in: Medicines (Basel, Switzerland) Vol. 6; no. 4; p. 101
• Main Authors: Cholet, Juliette, Decombat, Caroline, Vareille-Delarbre, Marjolaine, Gainche, Maël, Berry, Alexandre, Senejoux, François, Ripoche, Isabelle, Delort, Laetitia, Vermerie, Marion, Fraisse, Didier, Felgines, Catherine, Ranouille, Edwige, Berthon, Jean-Yves, Priam, Julien, Saunier, Etienne, Tourrette, Albert, Troin, Yves, Thebaud, Gilles, Chalard, Pierre, Caldefie-Chezet, Florence
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI 11-10-2019; MDPI AG
• Subjects: antioxidant; bupleurum rotundifolium; Chemical Sciences; cytokines; inflammation; pbmcs; antioxidant; cytokines; Bupleurum rotundifolium; inflammation; PBMCs; antioxidan
• ISSN: 2305-6320; 2305-6320
• DOI: 10.3390/medicines6040101

Some Bupleurum species, such as the
DC. or the
Willd have been extensively studied (especially their roots) for the treatment of inflammation. In contrast, only compounds extracted from the aerial parts of
have been studied and showed anti-inflammatory or antiproliferative activities. This study was conducted to investigate the antioxidant, anti-inflammatory, and immunomodulatory effects of
roots.
To tackle the various aspects of inflammation, we studied in vitro a methanolic extract from the roots of
on peripheral blood mononuclear cells (PBMCs), polymorphonuclear neutrophils (PMNs), and the monocytic cells THP-1. Its antioxidant capacities and iron-chelating activity were assessed. The extract was tested on THP-1 differentiation, reactive oxygen species (ROS) production by leukocytes, neutrophils chemotaxis, cytokines, PGE2 production, and NF-κB activation in PBMCs.
The extract showed a decreased ROS production in stimulated cells. It increased PBMC chemokine secretion and up-regulated the differentiation of THP-1 monocytes into macrophage-like cells, indicating a potential interest of the extract in the resolution of acute inflammation. In addition, the analysis of cytokine production suggests that
has immunomodulatory properties.
Cytokines secretion, especially IL-1β and IL-12p70, provided us with a set of indicators suggesting that the extract might be able to drive the polarization of macrophages and lymphocytes toward a Th2 anti-inflammatory profile in excessive inflammation.