Preparation and characterization of bFGF and BSA-loaded microspheres

• Published in: Journal of drug delivery science and technology Vol. 15; no. 5; pp. 371 - 375
• Main Authors: Cetin, M., Capan, Y., Vural, I., Dogan, A.L., Guc, D., Hincal, A.A., Wehrlé, P., Dalkara, T.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 2005
• Subjects: bFGF; BSA; Microspheres; PLGA; SDS-PAGE; Microspheres; BSA; SDS-PAGE; PLGA; bFGF
• ISSN: 1773-2247
• DOI: 10.1016/S1773-2247(05)50067-4

This article describes the preparation and characterization of BSA and bFGF-loaded PLGA microspheres and assesses the effect of entrapment procedure and in vitro release conditions. In the experiments, changing the PVA concentration from 3 to 5% (w/v) in the pre-emulsion inner phase led to a change in particle size and encapsulation efficiency of BSA-loaded and bFGF-loaded PLGA microspheres from 1 to 0.7 μm and from 73 to 65%, respectively. The in vitro release of the bFGF from PLGA microspheres was much lower than BSA-loaded PLGA microspheres. For all of the formulations, BSA and bFGF retained integrity after the microencapsulation process as evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Besides, the cell proliferation-stimulating activity of bFGF was evaluated by using BALB/c 3T3 cells and it was shown that the bioactivity of bFGF was increased by adding heparin to the release medium.