Comparative analysis of the structure and thermal stability of sea urchin peristome and rat tail tendon collagen

We have purified collagen from two distinct sources; the vertebrate, rat tail tendon and an invertebrate, sea urchin adult tissue, the peristome. The collagenous nature of the purification products was confirmed by amino acid compositional analysis. Both preparations had high contents of glycine and...

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Published in:Journal of cellular biochemistry Vol. 84; no. 3; pp. 567 - 574
Main Authors: Mayne, Janice, Robinson, John J.
Format: Journal Article
Language:English
Published: New York John Wiley & Sons, Inc 2002
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Summary:We have purified collagen from two distinct sources; the vertebrate, rat tail tendon and an invertebrate, sea urchin adult tissue, the peristome. The collagenous nature of the purification products was confirmed by amino acid compositional analysis. Both preparations had high contents of glycine and proline residues and hydroxyproline was also present. The total pyrrolidine (proline + hydroxyproline) content decreased from 17.9 mole % in rat tail collagen to 12.9 mole % in peristome collagen. Distinctly different circular dichroic spectra were measured for these collagens. Analyses of spectra, measured as a function of temperature, revealed distinct thermal denaturation profiles. The melting temperature for rat tail collagen was 38.5°C, while the corresponding value for peristome collagen was significantly lower at 27°C. A similar thermal denaturation profile was obtained for rat tail collagen in digestion experiments using a 41‐kDa gelatinase activity, isolated from sea urchin eggs. These results identify structural differences between a typical, vertebrate type I fibrillar collagen and an echinoderm collagen which serves as a constituent of a mutable connective tissue. These differences may relate to the functional roles played by collagen in these distinctly different tissues. J. Cell. Biochem. 84: 567–574, 2002. © 2001 Wiley‐Liss, Inc.
Bibliography:Natural Sciences and Engineering Research Council of Canada
istex:05CE72D5D04EF3362D52341134146378CF5EF1E4
ark:/67375/WNG-H4KFHG60-T
ArticleID:JCB10048
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.10048