Porphyran-capped silver nanoparticles as a promising antibacterial agent and electrode modifier for 5-fluorouracil electroanalysis

• Published in: Carbohydrate research Vol. 498; p. 108193
• Main Authors: Bojko, Luana, de Jonge, Gabriela, Lima, Dhésmon, Lopes, Luma Clarindo, Viana, Adriano Gonçalves, Garcia, Jarem Raul, Pessôa, Christiana Andrade, Wohnrath, Karen, Inaba, Juliana
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-12-2020
• Subjects: 5-Fluorouracil; Antimicrobial properties; Green synthesis; Porphyran; Silver nanoparticles; Square wave voltammetry; Green synthesis; Square wave voltammetry; Antimicrobial properties; 5-Fluorouracil; Porphyran; Silver nanoparticles
• ISSN: 0008-6215; 1873-426X
• DOI: 10.1016/j.carres.2020.108193

In the present work, the green synthesis of silver nanoparticles (AgNPs) using the sulfated polysaccharide porphyran (PFR) as capping agent and d-glucose as reducing agent is described. PFR was extracted from red seaweed and characterized by employing 13C NMR and determination of total sugar, protein, and sulfate contents. The obtained AgNPs-PFR were characterized by using UV-VIS spectroscopy, zeta potential determination, FESEM, and TEM, which demonstrated that PFR was effective at capping the AgNPs, yielding stable suspensions. The AgNPs-PFR presented good antimicrobial properties against Gram-positive and Gram-negative bacterial strains (Staphylococcus aureus and Escherichia coli, respectively). The AgNPs-PFR were also employed as the modifier of carbon paste electrodes, which were efficiently applied as electrochemical sensors for the determination of 5-fluorouracil (5-FU), an important anticancer drug, through square wave voltammetry (SWV). The AgNPs-PFR improved the electrochemical properties of the electrodes, and enhanced their electroanalytical performance. The developed sensing device presented detection and quantification limits equal to 10.7 and 35.8 μmol L-1, respectively, towards 5-FU determination. The proposed electrochemical sensor successfully quantified 5-FU in a real pharmaceutical formulation, confirming its potential as a new promising analytical detection tool for 5-FU quality control purposes.
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•The green synthesis of porphyran-capped silver nanoparticles (AgNPs-PFR) is reported.•Porphyran was extracted from red seaweed and characterized.•AgNPs-PFR showed good antibacterial properties.•AgNPs-PFR improved the electrochemical features of carbon paste electrodes.•The developed sensors were efficiently applied to 5-fluorouracil determination.