Direct Identification of Each Specific Mutation in Codon 12 and 13 of ci-ki-ras2 by SSCP Analysis

We compared the SSCP behaviour of the DNA fragments containing c-ki-ras 2 wild type 12 and 13 codons or each of the 12 possible point mutated sequences in these two codons. We found that a single electrophoresis condition was sufficient to distinguish each specific mutation from the other 11 and fro...

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Bibliographic Details
Published in:	Biochemical and biophysical research communications Vol. 246; no. 3; pp. 813 - 815
Main Authors:	Farina, Mario La, Maturi, Natascia, Stira, Salvatore, Russo, Antonio, Bazan, Viviana, Albanese, Ida
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 29-05-1998
Subjects:	Carcinoma - genetics Cloning, Molecular Codon Colorectal Neoplasms - genetics DNA, Neoplasm - isolation & purification Electrophoresis, Polyacrylamide Gel Gene Frequency Genes, ras Humans Mutation Polymorphism, Single-Stranded Conformational Proto-Oncogene Proteins p21(ras) - genetics
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Summary:	We compared the SSCP behaviour of the DNA fragments containing c-ki-ras 2 wild type 12 and 13 codons or each of the 12 possible point mutated sequences in these two codons. We found that a single electrophoresis condition was sufficient to distinguish each specific mutation from the other 11 and from the wild type sequence. This observation makes it possible to identify each specific mutation directly by SSCP without any need for reamplification and sequencing.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0006-291X
DOI:	10.1006/bbrc.1998.8716