A novel application of Onyx™ monolithic column for simultaneous determination of salicylic acid and triamcinolone acetonide by sequential injection chromatography

• Published in: Talanta (Oxford) Vol. 72; no. 2; pp. 854 - 858
• Main Authors: Chocholouš, Petr, Holík, Pavel, Šatínský, Dalibor, Solich, Petr
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 30-04-2007; Oxford Elsevier
• Subjects: Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; ONYX™ monolithic column; Other chromatographic methods; Salicylic acid; Sequential injection chromatography, SIC; Spectrometric and optical methods; Spectrophotometric determination; Triamcinolone acetonide; Salicylic acid; Spectrophotometric determination; ONYX™ monolithic column; Sequential injection chromatography, SIC; Triamcinolone acetonide; Water; Peak resolution; Internal standard; HPLC chromatography; Phase analysis; Mobile phase; Sequential method; Detection limit; Standard deviation; Time analysis; Flow injection; Trace analysis; Correlation coefficient; Drug; Precolumn; Sequential injection chromatography; Dilution; Ultraviolet detector; Silicon carbide; Linearity; Acetonitrile; Simultaneous measurement; Acetic acid; Spectrophotometry; SIC; ONYX# monolithic column; Salicylic acid; Triamcinolone acetonide; Spectrophotometric determination
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2006.12.009

A novel and fast simultaneous determination of triamcinolone acetonide (TCA) and salicylic acid (SA) in topical pharmaceutical formulations by sequential injection chromatography (SIC) as an alternative to classical high performance liquid chromatography (HPLC) has been developed. A recently introduced Onyx™ monolithic C18 (50
mm
×
4.6
mm, Phenomenex
®) with 5
mm monolithic precolumn were used for the first time for creating sequential injection chromatography system based on a FIAlab
® 3000 with a six-port selection valve and 5.0
mL syringe pump in study. The mobile phase used was acetonitrile/water (35:65, v/v), pH 3.3 adjusted with acetic acid at flow rate 0.9
mL
min
−1. UV detection provided by fibre-optic DAD detector was set up at 240
nm. Propylparaben was chosen as suitable internal standard (IS). There is only simple pre-adjustment of the sample of topical solution (dilution with mobile phase) so the analysis is not uselessly elongated. Parameters of the method showed good linearity in wide range, correlation coefficient >0.999; system precision (relative standard deviation, R.S.D.) in the range 0.45–1.95% at three different concentration levels, detection limits (3
σ) 1.00
μg
mL
−1 (salicylic acid), 0.66
μg
mL
−1 (triamcinolone acetonide) and 0.33
μg
mL
−1 (propylparaben) and recovery from the pharmaceutical preparations in the range 97.50–98.94%. The chromatographic resolution between peaks of compounds was more than 4.5 and analysis time was 5.1
min under the optimal conditions. The advantages of sequential injection chromatography against classical HPLC are discussed and showing that SIC can be a method of option in many cases.