Structure characterization and antitumor activity of an α β-glucan polysaccharide from Auricularia polytricha

• Published in: Food research international Vol. 45; no. 1; pp. 381 - 387
• Main Authors: Song, Guanglei, Du, Qizhen
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01-01-2012; Elsevier
• Subjects: Antitumor activity; Auricularia polytricha; Biological and medical sciences; Food industries; Fundamental and applied biological sciences. Psychology; Polysaccharide; Structure; Antitumor activity; Auricularia polytricha; Structure; Polysaccharide; Antineoplastic agent; Characterization; Glucan
• ISSN: 0963-9969; 1873-7145
• DOI: 10.1016/j.foodres.2011.10.035

A fraction of a 0.05M NaOH solution-soluble polysaccharide, termed AAFRC, was obtained from Auricularia polytricha by protease-assisted aqueous extraction followed by precipitation with ethanol and purification with gel filtration chromatography. Analysis by high performance liquid chromatography (HPLC) coupled to a TSK-G5000PWXL column and gel filtration chromatography on Sephacryl S-400HR indicated that AAFRC is homogenous with an average molecular weight (Mw) of about 1.20×106Da. The structure of AAFRC was revealed by chemical methods including Fourier transform infrared (FT-IR) spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy. Results indicated that AAFRC is a glucan consisting 1,3-β-glucan, 1,4-α-glucan, and 1,3-α-glucan backbone with a single 1→)-α-d-glucopyranosyl side-branching unit on every six residues, on average, along the main chain. The growth of transplantable Sarcoma-180 (S180) in mice was also significantly inhibited by AAFRC compared with the model controls (P<0.01), with the inhibitory rate being 43.61%.
► A novel α β-glucan polysaccharide with an average molecular weight of 1.20×106Da. ► The polysaccharide with a single side-branching unit on every six residues. ► The polysaccharide is able to significantly inhibit the growth of Sarcoma-180. ► The structure is useful for revealing mechanisms of food-based therapies.