CYCLOHEXENE NUCLEIC ACIDS (CeNA) FORM STABLE DUPLEXES WITH RNA AND INDUCE RNASE H ACTIVITY

• Published in: Nucleosides, nucleotides & nucleic acids Vol. 20; no. 4-7; pp. 785 - 788
• Main Authors: Wang, J., Verbeure, B., Luyten, I., Froeyen, M., Hendrix, C., Rosemeyer, H., Seela, F., Van Aerschot, A., Herdewijn, P.
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis Group 01-04-2001
• Subjects: Circular Dichroism; Cyclohexanes - chemistry; Cyclohexanes - metabolism; Cyclohexenes; DNA - chemistry; Enzyme Activation; Escherichia coli - enzymology; Nucleic Acids - chemistry; Nucleic Acids - metabolism; Ribonuclease H - metabolism; RNA - chemistry; RNA - metabolism; Sugar Alcohols - chemistry
• ISSN: 1525-7770; 1532-2335
• DOI: 10.1081/NCN-100002430

Cyclohexene nucleic acids (CeNA) were synthesized using classical phosporamidite chemistry. Incorporation of a cyclohexene nucleo-side in a DNA chain leads to an increase in stability of the DNA/RNA duplex. CeNA is stable against degradation in serum. A CeNA/RNA hybrid is able to activate E. Coli RNase H, resulting in cleavage of the RNA strand.