Species-specific repetitive DNA used to identify interspecific somatic hybrids

Plasmid DNA clones containing repetitive DNA sequences were isolated from Hyoscyamus muticus and Nicotiana tabacum. Non cross-hybridizing probes from each species were used in a simple hybridization test with DNA isolated from presumptive somatic hybrids. This allowed unequivocal identification of D...

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Bibliographic Details
Published in:	Plant cell reports Vol. 3; no. 2; pp. 65 - 67
Main Authors:	Saul, M.W, Potrykus, I
Format:	Journal Article
Language:	English
Published:	Berlin Springer 01-04-1984
Subjects:	Agronomy. Soil science and plant productions Biological and medical sciences Biotechnology Cell cultures. Hybridization. Fusion Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Genetics and breeding of economic plants Haploidy, in vitro culture applications, somatic hybrids Hyoscyamus muticus In vitro embryo culture Methods. Procedures. Technologies Molecular and cellular biology Nicotiana tabacum Plant breeding: fundamental aspects and methodology Plant cells and fungal cells tobacco Cell culture Molecular hybridization Vegetals Repeated sequence Identification Species specificity Cell fusion Nicotiana tabacum Plasmid Dicotyledones DNA Angiospermae Interspecific hybridization Somatic hybrid Spermatophyta Solanaceae
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Summary:	Plasmid DNA clones containing repetitive DNA sequences were isolated from Hyoscyamus muticus and Nicotiana tabacum. Non cross-hybridizing probes from each species were used in a simple hybridization test with DNA isolated from presumptive somatic hybrids. This allowed unequivocal identification of DNA from both species in the hybrids.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0721-7714 1432-203X
DOI:	10.1007/BF00270973