Quality analysis of selective microparticle deposition on electrically programmable surfaces

• Published in: Review of scientific instruments Vol. 81; no. 7; p. 073703
• Main Authors: Wagner, J, Löffler, F, König, K, Fernandez, S, Nesterov-Müller, A, Breitling, F, Bischoff, F R, Stadler, V, Hausmann, M, Lindenstruth, V
• Format: Journal Article
• Language: English
• Published: United States 01-07-2010
• Subjects: Electricity; Image Processing, Computer-Assisted - methods; Protein Array Analysis; Quality Control; Surface Properties
• ISSN: 1089-7623
• DOI: 10.1063/1.3456986

Image processing and pattern analysis can evaluate the deposition quality of triboelectrically charged microparticles on charged surfaces. The image processing method presented in this paper aims at controlling the quality of peptide arrays generated by particle based solid phase Merrifield combinatorial peptide synthesis. Incorrectly deposited particles are detected before the amino acids therein are coupled to the growing peptide. The calibration of the image acquisition is performed in a supervised training step in which all parameters of the quality analyzing algorithm are learnt given one representative image. Then, the correct deposition pattern is determined by a linear support vector machine. Knowing the pattern, contaminated areas can be detected by comparing the pattern with the actual deposition. Taking into account the resolution of the image acquisition system and its magnification factor, the number and size of contaminating particles can be calculated out of the number of connected foreground pixels.