Preparation of SF/SF-nHA double-layer scaffolds for periodental tissue regeneration

• Published in: International journal of polymeric materials Vol. 72; no. 18; pp. 1445 - 1454
• Main Authors: Guo, Ying, Jiang, Xuefeng, Pan, Peng, Liu, Xueping, Huang, Linling, Li, Mingzhong, Liu, Yu
• Format: Journal Article
• Language: English
• Published: Philadelphia Taylor & Francis 12-12-2023; Taylor & Francis Ltd
• Subjects: Compressive strength; Elongation; Hydroxyapatite; periodontal tissue; Pore size; Regeneration (physiology); Scaffolds; Silk fibroin; Tensile strength; Tissue engineering
• ISSN: 0091-4037; 1563-535X
• DOI: 10.1080/00914037.2022.2100375

Silk fibroin (SF) film/porous SF-nanohydroxyapatite (nHA) double-layer composite scaffolds were fabricated by a combination of electrospinning, casting and freeze-drying. The results showed that the tensile strength and elongation of the SF film layer were significantly improved through the bridging effect of SF/(ε-caprolactone) fibers in the SF matrix. The pore size in SF-nHA porous scaffold layer was approximately 213 μm, and nHA enhanced the compressive strength of the scaffold. CCK-8 assay showed that the SF/SF-nHA scaffold not only had no obvious cytotoxicity but also significantly promoted cell proliferation. The scaffold has the potential to be used for periodontal tissue regeneration.