Increased frequency of recognition of delipidated versus intact CNS myelin in multiple sclerosis and control subjects

• Published in: Journal of the neurological sciences Vol. 166; no. 1; pp. 23 - 27
• Main Authors: Trotter, John L, Keadle, Tammie L, Lauber, Joanne M, Dong, Wan Li, Cross, Anne H
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 15-06-1999; Elsevier Science
• Subjects: Adult; Biological and medical sciences; Case-Control Studies; Cell Division - physiology; Central Nervous System - metabolism; Central nervous system myelin; Female; Humans; Leukocytes, Mononuclear - cytology; Leukocytes, Mononuclear - metabolism; Lipid; Lymphocytes; Male; Medical sciences; Membrane Lipids - metabolism; Middle Aged; Multiple sclerosis; Multiple Sclerosis - metabolism; Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis; Myelin Basic Protein - metabolism; Myelin Proteolipid Protein - metabolism; Myelin Sheath - physiology; Neurology; T-Lymphocytes - immunology; Central nervous system myelin; Multiple sclerosis; Lymphocytes; Lipid; Human; Nervous system diseases; Myelin; Pathogenesis; Exploration; Lipids; Proliferation; Inflammatory disease; Delipidation; Mononuclear cell; Central nervous system disease; T-Lymphocyte; Adult; Comparative study
• ISSN: 0022-510X; 1878-5883
• DOI: 10.1016/S0022-510X(99)00106-9

The proliferative response of mononuclear cells from MS patients and normal control subjects to intact and delipidated myelin membranes was examined. The mean frequency of recognition in both groups of human subjects was greater for delipidated myelin than for intact myelin. Human T cell lines established using intact or delipidated myelin as the antigen were highly heterogeneous in response, and were each able to recognize myelin basic protein and myelin proteolipid protein peptides. However, there was no difference in the frequency of recognition of either form of myelin membrane when MS patients were compared to control subjects. Our results suggest that the presentation of delipidated forms of membrane proteins might enhance the response to myelin antigens in vivo, and be relevant to demyelinating diseases.