Antimalarial and Immunomodulatory Activities of Tithonia diversifolia (Asteraceae) Leave Flafonoids-Rich Extract Used in Cameroonian Traditional Medecine

• Published in: Scientifica (Cairo) Vol. 2024; no. 1; p. 8645178
• Main Authors: Ngum, Ntonifor Helen, Masakebenagha, Ndoah Ellen, Mahamat, Oumar
• Format: Journal Article
• Language: English
• Published: Egypt Wiley 2024; Hindawi Limited
• ISSN: 2090-908X; 2090-908X
• DOI: 10.1155/2024/8645178

Phytochemicals are considered the reliable source for the treatment of infection including malaria. Especially, phenols are known as potentially toxic to the growth and development of pathogens, among which flavonoids are the most extensively studied and play more intensive roles in ethnopharmacology. The immunological effect and role of
flavonoids-rich extract in treatment of malaria have therefore been examined in this study.
test against
and 4-day suppressive and Rane's tests against
in mice were used to evaluate the antimalarial activities. TNF-
and INF-
levels, phagocytic tests, and production of oxygen and nitrogen radical were assessed to appreciate the immunomodulatory activity. One-way analysis of variance followed by post hoc Student's
tests was used for data analysis.
flavonoids-rich extract at the concentrations ranging from 0.0004 mg/ml significantly (
< 0.05) inhibited in a concentration-dependent manner the growth of trophozoite up to 100% inhibition with 0.025 mg/ml at 24 and 48 hrs. Moreover,
flavonoids-rich extract reduced the level of parasitemia and improved in a dose-dependent manner the survival time of infected mice significantly (
< 0.05) compared to their control in 4-day suppressive test as well as in Rane's test. Additionally,
flavonoids-rich extract increased the TNF-
and INF-
levels in rats infected by
. Furthermore, the flavonoid-rich extract enhanced weight of spleen in the rats, the metabolic and phagocytic activities of the peritoneal cells, and the concentration of nitric oxide and oxygen radicals in methylprednisolone-immunocompromised rats compared to the control (
< 0.05).
The study has revealed that
flavonoids-rich extract through its antiplasmodial and phagocytic activities is a promising treatment of malaria.