Callus induction and plant regeneration from maize mature embryos

Calli were induced from mature embryos of maize (Zea mays L.) inbred lines A632, B73 and Mol7 on MS medium supplemented with 1-2 mg/1 2,4-dichlorophenoxyacetic acid. Callus induction frequency ranged from 23-100%, with Mol7 having the highest frequency. Plants were regenerated from 4-5% of the B73 a...

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Bibliographic Details
Published in:	Plant cell reports Vol. 6; no. 5; pp. 360 - 362
Main Author:	Wang, A.S
Format:	Journal Article
Language:	English
Published:	Berlin Springer 01-10-1987
Subjects:	Agronomy. Soil science and plant productions Biological and medical sciences Biotechnology callus Cell cultures. Hybridization. Fusion Economic plant physiology embryo (plant) Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology In vitro culture In vitro embryo culture Methods. Procedures. Technologies Molecular and cellular biology Plant cells and fungal cells regeneration tissue culture Zea mays Embryonic development Regeneration Monocotyledones Callus Vegetals Zea mays Gramineae Embryo culture Angiospermae Spermatophyta Cereal crop Organogenesis
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Summary:	Calli were induced from mature embryos of maize (Zea mays L.) inbred lines A632, B73 and Mol7 on MS medium supplemented with 1-2 mg/1 2,4-dichlorophenoxyacetic acid. Callus induction frequency ranged from 23-100%, with Mol7 having the highest frequency. Plants were regenerated from 4-5% of the B73 and Mol7 explants. Embryogenic and organogenic calli of B73 were maintained for more than two and one half years without losing regenerability. Of 95 regenerated plants, only one R0 plant with abnormal pollen was detected, and no morphological variants were observed in the R1 progeny.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0721-7714 1432-203X
DOI:	10.1007/BF00269560