Different approaches for multi-transgene-stacking in plants

• Published in: Plant science (Limerick) Vol. 163; no. 2; pp. 281 - 295
• Main Authors: François, Isabelle E.J.A, Broekaert, Willem F, Cammue, Bruno P.A
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01-08-2002; Elsevier Science
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic engineering applications; Genetic technics; Genetic transformation; Genetics and breeding of economic plants; Methods. Procedures. Technologies; Multi-transgene stacking; Plant biotechnology; Plant breeding: fundamental aspects and methodology; Transgenic animals and transgenic plants; Transgenic plants; Genetic transformation; Multi-transgene stacking; Plant biotechnology; Biotechnology; Polygenic; Transgenic plant; Metabolic pathway; Review; Gene expression; Genome
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/S0168-9452(02)00130-9

The introduction and expression of several transgenes is frequently required in either basic or applied studies in plant biotechnology. For instance, the expression of polygenic agronomic characteristics in plants or the manipulation of complex biosynthetic pathways requires introduction of multiple transgenes into the plant genome. Hence, full exploitation of the potential for manipulating plant metabolism awaits the development and adoption of methods for the routine introduction or manipulation of multiple transgenes. This review describes the generation of plants with multiple transgenes and summarizes our current understanding of the different approaches for obtaining these transgenic plants.