Induction, development and germination of somatic embryos from nucellar tissues of cashew ( Anacardium occidentale L.)

• Published in: Scientia horticulturae Vol. 93; no. 3; pp. 367 - 372
• Main Authors: Cardoza, Vinitha, D’Souza, L.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 19-04-2002; Elsevier
• Subjects: Biological and medical sciences; Biotechnology; Cashew; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; Germination; In vitro propagation: entire plant regeneration from tissues and cell cultures; Methods. Procedures. Technologies; Nucellus; Picloram; Plant cells and fungal cells; Putrescine; Somatic embryos; Nucellus; Picloram; Putrescine; Cashew; Germination; Somatic embryos; Sucrose; Anacardium occidentale; Tissue culture; Callogenesis; Disaccharide; Pyridine derivatives; Somatic embryo; Dicotyledones; Angiospermae; Nucella; Development; Chemical composition; Fruit tree; Anacardiaceae; Plant tissue; Culture medium; Polyamine; Micropropagation; picolinic acid derivative; Regeneration; Spermatophyta; Embryonic induction
• ISSN: 0304-4238; 1879-1018
• DOI: 10.1016/S0304-4238(01)00348-X

Fully developed somatic embryos were induced from nucellar callus in cashew. Nucellar tissue cultured on MS medium with 0.5
mg/l picloram with 20
g/l sucrose gave rise to embryogenic callus and proembryos. The proembryos when cultured on MS medium with 0.5
mg/l picloram and 1
mg/l putrescine gave rise to globular embryos. Globular embryos matured to form heart-shaped and well-developed cotyledonary stage embryos on MS medium with 30
g/l sucrose and 0.5
mg/l ABA. The mature embryos germinated on MS medium devoid of growth regulators.