A total internal reflection fluorescence biosensor for aluminum (III)

• Published in: Microchemical journal Vol. 70; no. 1; pp. 63 - 68
• Main Authors: Qian, Fang, Asanov, Alexander N, Oldham, Philip B
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier B.V 01-09-2001; Elsevier
• Subjects: Aluminum(III); Biological and medical sciences; Biosensor; Biosensors; Biotechnology; FITC; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Phosvitin; TIRF; Various methods and equipments; FITC; Aluminum(III); Phosvitin; Biosensor; TIRF; Aluminium Complexes; Performance evaluation; Total internal reflection; Fluorescence detector; Phosphoproteins; Concentration effect; Luminescence quenching; Organic ligand; Metal Complexes
• ISSN: 0026-265X; 1095-9149
• DOI: 10.1016/S0026-265X(00)00162-4

Total internal reflection fluorescence (TIRF) is widely used for investigating interfacial interactions. It has proven to be a well-suited technique for biosensing applications due to its surface sensitivity and minimal sample consumption. In this paper, the protein phosvitin has been investigated as a molecular sensor for the detection of aluminum (III). Phosvitin was labeled with fluorescein isothiocyanate (FITC) and immobilized on a quartz surface via EDC/NHS chemistry. Phosvitin–FITC and aluminum complexation, both in free solution and surface-bound form, was studied. A TIRF biosensor selective for Al
3+ was constructed using surface-immobilized phosvitin–FITC. An enhancement of phosvitin–FITC fluorescence intensity was observed upon addition of Al
3+. The response was linear over the range 0.1–10 μM of aluminum concentration.