An integrative GC-MS and LC-MS metabolomics platform determination of the metabolite profile of Bombax ceiba L. root, and in silico & in vitro evaluation of its antibacterial & antidiabetic activities

• Published in: Natural product research Vol. 37; no. 13; pp. 2263 - 2268
• Main Authors: Alrabie, Ali, Alrabie, Nabeel Abdullah, AlSaeedy, Mohammed, Al-Adhreai, Arwa, Al-Qadsy, Inas, Al-Horaibi, Sultan A, Alaizeri, ZabnAllah M, Alhadlaq, Hisham A, Ahamed, Maqusood, Farooqui, Mazahar
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis Ltd 01-07-2023
• Subjects: Ampicillin; Antibacterial activity; Bombax ceiba; Chemical composition; Chemical compounds; Diabetes mellitus; Glucosidase; Metabolites; Metabolomics; Methanol; Minimum inhibitory concentration; Molecular docking; Roots; α-Amylase; α-Glucosidase; Bombax ceiba L; asiatic acid; cedrene; lathodoratin; GC–MS analysis; LC–MS analysis
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2022.2149519

The
L. tree is a member of the family
and the genus Bombax. Both Chinese and Indian traditional medicine have made extensive use of it in the treatment of sickness. Its chemical composition is still a mystery.
roots methanol extract (BCRME) was analyzed by different chromatographic analytical techniques in order to identify its major chemical constituents. Twelve compounds and six compounds were identified from GC-MS and LC-MS analysis, respectively. This is the first report on the presence of lathodoratin, cedrene, 4H-1-benzopyran-4-one,8-[{dimethylamino} methyl]-7-methoxy-3-methyl-2-phenyl, asiatic acid, and (E)-2,4,4'-trihydroxylchalcone in
roots. Methanol extract demonstrated noteworthy antibacterial activity against
(MTCC96) (MIC: 100 µg/mL) compare to antibiotic ampicillin (MIC: 250 µg/mL) as well as the highest α-amylase inhibition (IC
=26.91 µg/mL) and α-glucosidase inhibition (IC
=21.21 µg/mL) effects, molecular docking study confirmed these findings, with some compounds having a very high docking score.