An ultra-low noise amplifier array system for high throughput single entity analysis

• Published in: Faraday discussions Vol. 233; p. 33
• Main Authors: Zhong, Cheng-Bing, Ma, Hui, Wang, Jia-Jun, Zhang, Lin-Lin, Ying, Yi-Lun, Wang, Rong, Wan, Yong-Jing, Long, Yi-Tao
• Format: Journal Article
• Language: English
• Published: England 05-04-2022
• Subjects: Catalysis; Electrochemistry; Electrodes; Nanopores; Nanotechnology
• ISSN: 1364-5498
• DOI: 10.1039/d1fd00055a

Electrochemical measurements at the single entity level provide ultra-sensitive tools for the precise diagnosis and understanding of basic biological and chemical processes. By decoding current signatures, single-entity electrochemistry provides abundant information on charges, sizes, shapes, catalytic performances and compositions. The accuracy of single-entity electrochemistry highly relies on advanced instrumentation to achieve the amperometric resolution at the sub-picoampere level and the temporal resolution at the sub-microsecond level. Currently, it is still a challenge for paralleling amplifiers to allow low-noise and high bandwidth single-entity electrochemical measurements. Herein, we developed a low-noise four-channel electrochemical instrumentation that integrates an Au electrode array with amplifiers in the circuit board. With this amplifier array, we achieved a high bandwidth (>100 kHz) electrochemical measurement. The further practical experiments proved the capability of this amplifier array system in acquiring transient signals from both single-molecule detection with an aerolysin nanopore and single Pt nanoparticle catalysis during the dynamic collision process. Paired with appropriate microfluidic array systems, our instrumentation will enable an extraordinarily high-throughput feature for single-entity sensing.