Effects of nesfatin-1 on food intake and LH secretion in prepubertal gilts and genomic association of the porcine NUCB2 gene with growth traits

• Published in: Domestic animal endocrinology Vol. 45; no. 2; pp. 89 - 97
• Main Authors: Lents, C.A, Barb, C.R, Hausman, G.J, Nonneman, D, Heidorn, N.L, Cisse, R.S, Azain, M.J
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-08-2013
• Subjects: adiponectin; Adipose Tissue - chemistry; Adipose Tissue - metabolism; adiposity; Adiposity - genetics; adults; agonists; alpha-MSH - analogs & derivatives; alpha-MSH - pharmacology; Amino Acid Sequence; Animals; appetite; Appetite Regulation - physiology; barrows; Calcium-Binding Proteins - genetics; Calcium-Binding Proteins - pharmacology; Calcium-Binding Proteins - physiology; DNA-Binding Proteins - genetics; DNA-Binding Proteins - pharmacology; DNA-Binding Proteins - physiology; Eating - drug effects; Eating - physiology; energy; feed intake; Female; food intake; gene expression; Gene Expression - drug effects; genes; gilts; growth traits; homeostasis; hormone secretion; Humans; hypothalamus; Hypothalamus - chemistry; Injections, Intraventricular; leptin; luteinizing hormone; Luteinizing Hormone - secretion; messenger RNA; Molecular Sequence Data; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - pharmacology; Nerve Tissue Proteins - physiology; nicotinamide phosphoribosyltransferase; phenotype; Polymorphism, Single Nucleotide - genetics; puberty; Receptor, Melanocortin, Type 4 - agonists; RNA, Messenger - analysis; Sequence Alignment; Sexual Maturation - physiology; single nucleotide polymorphism; subcutaneous fat; Sus scrofa - growth & development; Sus scrofa - physiology; Luteinizing hormone; Porcine; Nesfatin-1; Nucleobindin 2; Feed intake; Polymorphism
• ISSN: 0739-7240; 1879-0054
• DOI: 10.1016/j.domaniend.2013.06.002

Nesfatin-1, a product of the nucleobindin 2 (NUCB2) gene, purportedly plays important roles in whole-body energy homeostasis. Experiments were conducted to determine how NUCB2 expression in fat depots may be controlled in the pig and to test the hypothesis that nesfatin-1 regulates appetite and LH secretion in the gilt. Prepubertal gilts were used to study expression of NUCB2 in fat and the effects of intracerebroventricular (i.c.v.) injection of nesfatin-1 on food intake and pituitary hormone secretion. Growing pigs (gilts and barrows at 22 wk of age, n = 1,145) or sexually mature gilts (n = 439) were used to test association of SNP in the NUCB2 gene with growth traits. The expression of NUCB2 was similar for subcutaneous fat compared with perirenal fat. An i.c.v. injection of the melanocortin-4 receptor agonist [Nle⁴, d-Phe⁷]-α-melanocyte-stimulating hormone did not alter expression of NUCB2 mRNA in the hypothalamus but reduced (P = 0.056) NUCB2 mRNA expression in subcutaneous fat. Short-term (7 d) submaintenance feeding reduced (P < 0.05) BW and did not alter expression of mRNA for NUCB2, visfatin, or leptin but increased (P < 0.05) expression of adiponectin mRNA in fat. Central injection of nesfatin-1 suppressed (P < 0.001) feed intake. Secretion of LH was greater (P < 0.01) after i.c.v. injection of nesfatin-1 than after saline. Single nucleotide polymorphisms in the porcine NUCB2 gene were not associated with adiposity of growing pigs or age at puberty in gilts but were associated (P < 0.05) with BW at puberty. These data indicate that NUCB2 is expressed in fat depots of the pig and that the level of expression is sensitive to stimulation of appetite-regulating pathways in the hypothalamus. It is confirmed herein that nesfatin-1 can regulate appetite in the pig and affect the gonadotropic axis of the prepubertal pig. Association of SNP in the porcine NUCB2 gene with BW at puberty suggests that regulation of appetite by nesfatin-1 in the pig affects growth, which may have important consequences for adult phenotypes.