All species of the genus Bordetella contain genes for pertussis toxin of Bordetella pertussis

• Published in: Zentralblatt für Bakteriologie, Mikrobiologie und Hygiene. Series A, Medical microbiology, infectious diseases, virology, parasitology Vol. 269; no. 2; p. 205
• Main Authors: Rozinov, M N, Dain, A A, Shumakov, Y L, Lapaeva, I A, Holzmayer, T A
• Format: Journal Article
• Language: English
• Published: Germany 01-08-1988
• Subjects: Blotting, Southern; Bordetella - genetics; Bordetella pertussis - genetics; Cloning, Molecular; Deoxyribonuclease EcoRI; Deoxyribonucleases, Type II Site-Specific; DNA Probes; DNA Restriction Enzymes; DNA, Bacterial - genetics; Genes, Bacterial; Nucleic Acid Hybridization; Pertussis Toxin; Plasmids; Virulence Factors, Bordetella - genetics
• ISSN: 0176-6724
• DOI: 10.1016/S0176-6724(88)80098-1

A molecular probe for the PT-operon of B. pertussis hybridized with 4.7 Kb EcoRI-fragments of chromosomal DNAs of B. pertussis strain 475 phase I, phase IV, B. parapertussis strains 504 and 17903, B. bronchiseptica strain 214, B. parapertussis strain 17903-convertant of B. pertussis phage 134 but not with phage 134 DNA under stringent conditions of DNA-DNA hybridization. This fact indicates the presence of PT-genes in all Bordetella species. Since there is no production of PT in B. parapertussis and B. bronchiseptica, a presence of regulatory mutations in the PT-operon or absence of the functionally active vir-gene product in these species is suggested.