The proteolytic fraction from Vasconcellea cundinamarcensis accelerates wound healing after corneal chemical burn in rabbits

• Published in: Burns Vol. 46; no. 4; pp. 928 - 936
• Main Authors: Oliveira Silva, Rummenigge, da Costa, Bruna Lopes, da Silva, Carolina Nunes, da Mata Martins, Thaís Maria, Nunes Dourado, Lays Fernanda, de Goes, Alfredo Miranda, Lopes, Miriam Teresa, Salas, Carlos Edmundo, Silva-Cunha, Armando da, da Silva, Flavia Rodrigues
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-06-2020
• Subjects: Chemical burn; Corneal healing; HET-CAM; P1G10; Vasconcellea cundinamarcensis; PBS; Corneal healing; EGF; Vasconcellea cundinamarcensis; NaOH; MALDI-MS; Chemical burn; MTT; IAA-P1G10; HET-CAM; HCl; MSCs; NSAIDs; CMC; SEM; P1G10; HPLC
• ISSN: 0305-4179; 1879-1409
• DOI: 10.1016/j.burns.2019.10.005

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•P1G10 is suitable for ocular administration.•P1G10 solution accelerates re-epithelialization in vivo study using chemical corneal burn in rabbits.•P1G10 modulates the inflammatory response after corneal injury.•P1G10 controls the arrangement of collagen fibers during the corneal wound healing.
Chemical ocular burns are among the most frequently eye-related injuries, which require immediate and intensive evaluation and care since they may lead to potential complications such as superinfection, corneal perforation, and blindness.Vasconcellea cundinamarcensis, a species from Caricaceae family, contains highly active proteolytic enzymes in its latex that show healing activity in animal models bearing lesions of different etiologies.
We evaluate the ocular toxicity of the proteolytic fraction from V. cundinamarcensis (P1G10) by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and Hen’s Egg Test-Chorioallantoic Membrane test. The corneal healing property of P1G10 was studied by the ethanol-chemical burn in the rabbit’s eyes.
P1G10 is safe for ocular administration, except when administrated at 10μg/mL. P1G10 at 1μg/mL accelerates the corneal re-epithelization achieving complete wound closure after 72h of chemical burn. Also, P1G10 modulated the inflammatory response and controlled the arrangement of collagen fibers in the stroma, demonstrating its potential corneal healing properties.
Our work was the first one to evaluate the ophthalmic application of P1G10. Here we demonstrated that P1G10 is suitable for ocular administration and it has a promising corneal healing activity which may emerge as a new pharmacological tool to the development of a new drug for ocular surface chemical injuries in the future.