Non-porous silica for ultrafast reversed-phase high-performance liquid chromatographic separation of aldehyde and ketone 2,4-dinitrophenylhydrazones

• Published in: Journal of Chromatography A Vol. 786; no. 1; pp. 47 - 55
• Main Authors: Pötter, Wilhelm, Lamotte, Stefan, Engelhardt, Heinz, Karst, Uwe
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 24-10-1997; Elsevier
• Subjects: Aldehydes; Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Derivatization; Exact sciences and technology; Ketones; Other chromatographic methods; Ketones; Derivatization; Aldehydes; LC; Reversed phase chromatography; HPLC chromatography; Temperature effect; Hydrazone; Aldehyde; Experimental study; Ketone; Ultraviolet visible spectrometry
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(97)00564-5

Ultrafast high-performance liquid chromatographic separation of aldehyde and ketone 2,4-dinitrophenylhydrazones was carried out on reversed-phase 1.5 μm non-porous silica. Separation times of DNPH and a series of 12 aldehyde and ketone derivatives or potentially interfering compounds were less than 4 min compared to approximately 15 min using conventional columns. Formation of the formaldehyde derivative in solution was monitored using injection frequencies of 30 s for 15 min. The temperature dependence of the separation showed differences between the hydrazones of aliphatic aldehydes and those of unsaturated aldehydes.