The transcriptional co-activator PGC-1α up regulates apelin in human and mouse adipocytes

• Published in: Regulatory peptides Vol. 150; no. 1; pp. 33 - 37
• Main Authors: Mazzucotelli, Anne, Ribet, Carole, Castan-Laurell, Isabelle, Daviaud, Danièle, Guigné, Charlotte, Langin, Dominique, Valet, Philippe
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier B.V 09-10-2008; Amsterdam Elsevier
• Subjects: Adenovirus; Adipokine; Apelin; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Microarray; PGC-1α; Subcutaneous adipose tissue; Vertebrates: endocrinology; PGC-1α; Subcutaneous adipose tissue; Adipokine; Microarray; Adenovirus; Apelin; Human; Adipocyte; Adipose tissue; Subcutaneous; Rodentia; Vertebrata; Mammalia; Mouse; Animal
• ISSN: 0167-0115; 1873-1686
• DOI: 10.1016/j.regpep.2008.04.003

By using pangenomic microarray, we identified apelin as a unique adipokine up regulated by the transcriptional co-activator peroxisome proliferator-activated receptor γ (PPARγ) co-activator 1α (PGC-1α) in human white adipocytes. We investigated its regulation
in vitro and
in vivo. Overexpression of PGC-1α by adenovirus in human adipocytes induces apelin expression and secretion. Pharmacological induction of cAMP, an upstream regulator of endogenous PGC-1α expression, up regulates apelin gene expression and also apelin secretion in human and mice adipocytes. Moreover, during cold exposure in mice, a physiological situation known to induce both cAMP and PGC-1α, apelin expression in adipocytes and plasma levels were increased. This is the first demonstration that PGC-1α is involved in the regulation of an adipokine gene expression and release.