Dynamic interactions between root NH4(+) influx and long-distance N translocation in rice: insights into feedback processes

• Published in: Plant and cell physiology Vol. 39; no. 12; pp. 1287 - 1293
• Main Authors: Kronzucker, H.J, Schjoerring, J.K, Erner, Y, Kirk, G.J.D, Siddiqi, M.Y, Glass, A.D.M
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01-12-1998
• Subjects: Absorption. Translocation of ions and substances. Permeability; Agronomy. Soil science and plant productions; Ammonium; ammonium compounds; Biological and medical sciences; chemical constituents of plants; Economic plant physiology; enzyme activity; Fundamental and applied biological sciences. Psychology; glutamate-ammonia ligase; Influx; N-13; nitrates; Nitrogen; Nitrogen stress; nutrient deficiencies; nutrient transport; nutrient uptake; Nutrition. Photosynthesis. Respiration. Metabolism; Oryza sativa; Plant physiology and development; Rice; roots; seedlings; shoots; Water and solutes. Absorption, translocation and permeability; Process dynamics; Monocotyledones; Ammonium; Long distance; Isotope labelling; Biological transport; Nitrogen Isotopes; Cereal crop; Oryza sativa; Regulation(control); Absorption; Spatial distribution; Gramineae; Angiospermae; Nutrient; Above ground plant part; Spermatophyta; Below ground plant part
• ISSN: 0032-0781; 1471-9053
• DOI: 10.1093/oxfordjournals.pcp.a029332

Ammonium influx into roots and N translocation to the shoots were measured in 3-week-old hydroponically grown rice seedlings (Oryza sativa L., cv. IR72) under conditions of N deprivation and NH4+ resupply, using 13NH4+ as a tracer. Root NH4+ influx was repressed in plants continuously supplied with NH4+ (at 0.1 mM), but a high proportion of absorbed N (20 to 30%) was translocated to the shoot in the form of N assimilates during the 13-min loading and desorption periods. Interruption of exogenous NH4+ supply for periods of 1 to 3 d caused NH4+ influx to be de-repressed. This same treatment caused N translocation to the shoot to decline rapidly, until, by 24 h, less than 5% of the absorbed 13N was translocated to the shoot, illustrating a clear priority of root over shoot N demand under conditions of N deprivation. Upon resupplying 1 mM NH4+, root NH4+ influx responded in a distinct four-phase pattern, exhibiting periods in which NH4+ influx was first enhanced and subsequently reduced. Notably, a 25 to 40% increase in root influx, peaking at approximately 2 h following re-exposure was correlated with a 4- to 5-fold enhancement in shoot translocation and a repression of root GS activity. The transient increase of NH4+ influx was also observed in seedlings continuously supplied with NO3- and subsequently transferred to NH4+. Extended exposure to NH4+ caused root NH4+ influx to decrease progressively, while shoot translocation was restored to approximately 30% of incoming NH4+. The nature of the feedback control of NH4+ influx as well as the question of its inducibility are discussed.