Protein immobilization capacity and covalent binding coverage of pulsed plasma polymer surfaces

• Published in: Applied surface science Vol. 256; no. 16; pp. 4984 - 4989
• Main Authors: Yin, Yongbai, Bax, Daniel, McKenzie, David R., Bilek, Marcela M.M.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01-06-2010; Elsevier
• Subjects: Condensed matter: electronic structure, electrical, magnetic, and optical properties; Condensed matter: structure, mechanical and thermal properties; Cross-disciplinary physics: materials science; rheology; Enzyme-linked immunosorbent assay; Exact sciences and technology; Physics; Plasma polymerized surface; Protein immobilization; SDS cleaning; Tropoelastin; X-ray photoelectron spectroscopy; Plasma polymerized surface; Protein immobilization; SDS cleaning; Tropoelastin; X-ray photoelectron spectroscopy; Enzyme-linked immunosorbent assay; Coverage rate; Polymers; X-ray photoelectron spectra
• ISSN: 0169-4332; 1873-5584
• DOI: 10.1016/j.apsusc.2010.03.013

Three carbon surfaces were deposited using pulsed plasma enhanced chemical vapour deposition method: a low and a high nitrogen-containing plasma polymer surfaces and a diamond-like carbon surface. The surfaces were analysed using both X-ray photoelectron spectroscopy (XPS) technique and the enzyme-linked immunosorbent assay (ELISA) method combining with sodium dodecyl sulphate (SDS) cleaning to investigate the capacity and covalent binding of the immobilized proteins. A good correlation was found on quantification of remaining protein after SDS cleaning using the ELISA method and the XPS technique. All surfaces had similar initial capacity of protein attachment but with large different resistance to SDS cleaning. The analysis showed that the high nitrogen-containing plasma polymer was the best biocompatible material due to its highest resistance to SDS cleaning, i.e. with the highest quantity (∼80%) of proteins bound covalently.