Modulation of the transcellular metabolism of 12(S)HETE by 10-11 reductase activity in cultured rat aortic smooth muscle cells

• Published in: Prostaglandins, leukotrienes and essential fatty acids Vol. 51; no. 3; p. 187
• Main Authors: Berard, A, Lacape, G, Daret, D, Larrue, J
• Format: Journal Article
• Language: English
• Published: Scotland 01-09-1994
• Subjects: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid; Animals; Aorta; Blood Physiological Phenomena; Cattle; Cell Division; Cells, Cultured; Chromatography, High Pressure Liquid; Culture Media - pharmacology; Hydroxyeicosatetraenoic Acids - metabolism; Membrane Lipids - metabolism; Muscle Proteins - metabolism; Muscle, Smooth, Vascular - cytology; Muscle, Smooth, Vascular - drug effects; Muscle, Smooth, Vascular - metabolism; Oxidoreductases - metabolism; Phospholipids - metabolism; Rats
• ISSN: 0952-3278
• DOI: 10.1016/0952-3278(94)90133-3

Cultured rat aortic smooth muscle cells (SMC) metabolize 12(S)hydroxyeicosatetraenoic acid (12(S)HETE) by two different pathways; beta-oxidation leading to 16:3(8-OH), and 10-11 reductase activity producing 20:3(12-OH) which is beta-oxidized to 16:2(8-OH). In this work, we demonstrate that 10-11 reductase activity is modulated in cultured rat aortic SMC as a function of cell state (proliferating vs quiescent) and stimulated by serum. Most of the 20:3(12-OH) is recovered in the incubation medium but a significant part is esterified into phospholipids. By comparison with its parent compound, 12(S)HETE, 20:3 (12-OH) is mainly incorporated into phosphatidyl-choline and phosphatidyl-ethanolamine, suggesting that it may affect cellular functions. Taken together, these findings may be relevant to the effects of 12(S)HETE on vascular SMC functions related to atherosclerotic development.