Taibaiella coffeisoli sp. nov., isolated from the soil of a coffee plantation

• Published in: International journal of systematic and evolutionary microbiology Vol. 66; no. 4; pp. 1627 - 1632
• Main Authors: Szabó, István, Szoboszlay, Sándor, Táncsics, András, Szerdahelyi, Soma G, Szu Cs, Ádám, Radó, Júlia, Benedek, Tibor, Szabó, László, Daood, Hussein G, Cserháti, Mátyás, Kriszt, Balázs
• Format: Journal Article
• Language: English
• Published: England 01-04-2016
• Subjects: Bacterial Typing Techniques; Bacteroidetes - classification; Bacteroidetes - genetics; Bacteroidetes - isolation & purification; Base Composition; Coffea; DNA, Bacterial - genetics; Fatty Acids - chemistry; Molecular Sequence Data; Phospholipids - chemistry; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Tanzania; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry; Tanzania
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.000873

A Gram-stain-negative, obligately aerobic, non-motile, non-sporulating, rod-shaped bacterium, designated TZCO2T, was isolated from the soil of an irrigated coffee plantation in Arusha, Tanzania, East Africa. Phylogenetic analysis, based on 16S rRNA gene sequences, indicated that the isolate is affiliated with the genus Taibaiella in the family Chitinophagaceae. Its closest relative is Taibaiella koreensis THG-DT86T (96.7%). The pH and temperature ranges for growth were pH 6.0-8.5 (optimum 7.0-7.5) and 10-35 °C (optimum 30 °C, respectively. The predominant fatty acids were iso-C15:0 (32.4%), iso-C15:1 G (22.6%), iso-C17:0 (15.1%) and iso-C17:0 3-OH (10.0%) The only isoprenoid quinone detected in strain TZCO2T was menaquinone-7 (MK-7); the major polar lipids were phosphoaminolipid, phosphatidylethanolamine, unidentified aminolipids and lipids. The DNA G+C content was 51.9 mol%. Physiological and chemotaxonomic data further confirmed that strain TZCO2T is distinct from other members of the genus Taibaiella. Thus, strain TZCO2T is considered to represent a novel species of the genus, for which the name Taibaiella coffeisoli sp. nov. is proposed. The type strain is TZCO2T (=NCAIM B 02601T=CCM 8601T).