Molecular Mechanisms of Biased and Probe-Dependent Signaling at CXC-Motif Chemokine Receptor CXCR3 Induced by Negative Allosteric Modulators

• Published in: Molecular pharmacology Vol. 93; no. 4; p. 309
• Main Authors: Brox, Regine, Milanos, Lampros, Saleh, Noureldin, Baumeister, Paul, Buschauer, Armin, Hofmann, Dagmar, Heinrich, Markus R, Clark, Timothy, Tschammer, Nuska
• Format: Journal Article
• Language: English
• Published: United States 01-04-2018
• Subjects: Acetamides - metabolism; Acetamides - pharmacology; Allosteric Regulation - drug effects; Allosteric Regulation - physiology; Dose-Response Relationship, Drug; HEK293 Cells; Humans; Molecular Docking Simulation - methods; Protein Binding - drug effects; Protein Binding - physiology; Pyrimidinones - metabolism; Pyrimidinones - pharmacology; Receptors, CXCR3 - antagonists & inhibitors; Receptors, CXCR3 - chemistry; Receptors, CXCR3 - metabolism; Signal Transduction - drug effects; Signal Transduction - physiology
• ISSN: 1521-0111
• DOI: 10.1124/mol.117.110296

Our recent explorations of allosteric modulators with improved properties resulted in the identification of two biased negative allosteric modulators, BD103 (
-1-{[3-(4-ethoxyphenyl)-4-oxo-3,4-dihydropyrido[2,3-
]pyrimi-din2yl]ethyl}-4-(4-fluorobutoxy)-
-[(1-methylpiperidin-4-yl)methyl}]butanamide) and BD064 (5-[(
-{1-[3-(4-ethoxyphenyl)-4-oxo-3,4-dihydropyrido[2,3-
]pyrimidin-2-yl]ethyl-2-[4-fluoro-3-(trifluoromethyl)phenyl]acetamido)methyl]-2-fluorophenyl}boronic acid), that exhibited probe-dependent inhibition of CXC-motif chemokine receptor CXCR3 signaling. With the intention to elucidate the structural mechanisms underlying their selectivity and probe dependence, we used site-directed mutagenesis combined with homology modeling and docking to identify amino acids of CXCR3 that contribute to modulator binding, signaling, and transmission of cooperativity. With the use of allosteric radioligand RAMX3 ([
H]
-{1-[3-(4-ethoxyphenyl)-4-oxo-3,4-dihydropyrido[2,3-
]pyrimidin-2-yl]ethyl}-2-[4-fluoro-3-(trifluoromethyl)phenyl]-
-[(1-methylpiperidin-4-yl)methyl]acetamide), we identified that F131
and Y308
contribute specifically to the binding pocket of BD064, whereas D186
solely participates in the stabilization of binding conformation of BD103. The influence of mutations on the ability of negative allosteric modulators to inhibit chemokine-mediated activation (CXCL11 and CXCL10) was assessed with the bioluminescence resonance energy transfer-based cAMP and
-arrestin recruitment assay. Obtained data revealed complex molecular mechanisms governing biased and probe-dependent signaling at CXCR3. In particular, F131
, S304
, and Y308
emerged as key residues for the compounds to modulate the chemokine response. Notably, D186
, W268
, and S304
turned out to play a role in signal pathway selectivity of CXCL10, as mutations of these residues led to a G protein-active but
-arrestin-inactive conformation. These diverse effects of mutations suggest the existence of ligand- and pathway-specific receptor conformations and give new insights in the sophisticated signaling machinery between allosteric ligands, chemokines, and their receptors, which can provide a powerful platform for the development of new allosteric drugs with improved pharmacological properties.