Effect of a neurostimulator on postnatal neurogenesis in rodent olfactory bulbs

• Published in: Neuropeptides (Edinburgh) Vol. 89; p. 102181
• Main Authors: Varentsov, Viacheslav E., Rumyanceva, Tatiana A., Verzilina, Anastasia D., Pshenisnov, Kirill K., Rudenko, Ekaterina E., Nikolenko, Vladimir N., Shevchuk, Ivan V., Sinelnikov, Mikhail Y.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01-10-2021; Elsevier Science Ltd
• Subjects: Animals; Cell Differentiation - physiology; Computer applications; Doublecortin; Doublecortin protein; Male; Nestin; Nestin - metabolism; Neurogenesis; Neurogenesis - physiology; Neurons - metabolism; Neuropeptides; Olfactory bulb; Olfactory Bulb - metabolism; Olfactory Bulb - physiology; Ontogenesis; Rats; Rats, Wistar; Ontogenesis; Nestin; Doublecortin; DCX; Olfactory bulb; Neurogenesis; OL; Nes
• ISSN: 0143-4179; 1532-2785
• DOI: 10.1016/j.npep.2021.102181

The aim of the study was to reveal the effect of neurostimulation with the TKPRPGP neuropeptide on the expression intensity of Doublecortin and Nestin in the olfactory bulb of white Wistar rats using immunohistochemical and computer analysis methods. An isolated assessment of early progenitor differentiation by the density of nestin-positive structures showed that stimulation from birth to 14 days preserves the level of nestin expression, preventing its decrease. When the administration of the neuropeptide is stopped, the expression of nestin decreases sharply, starting from the central zones of the bulb, and after three weeks it is no longer present. The dynamics of doublecortin positive structure density reflects an increase upon neuropeptide administration. Each course of neuropeptide administration caused an increase in the density of the marker, but the degree of effectiveness decreased with age, and the duration of the effect decreased. In conclusion, administration of the neuropeptide TKPRPGP to rats at an early age prolongs the expression of nestin and doublecortin in the olfactory bulbs of rats up to 35 days and up to 74 days of observation, respectively. The administration of the neuropeptide in adulthood does not lead to re-expression of these markers.