Alteromonas pelagimontana sp. nov., a marine exopolysaccharide-producing bacterium isolated from the Southwest Indian Ridge

• Published in: International journal of systematic and evolutionary microbiology Vol. 67; no. 10; pp. 4032 - 4038
• Main Authors: Sinha, Rupesh Kumar, Krishnan, K P, Singh, Archana, Thomas, Femi Anna, Jain, Anand, John Kurian, P
• Format: Journal Article
• Language: English
• Published: England 01-10-2017
• Subjects: Alteromonas - classification; Alteromonas - genetics; Alteromonas - isolation & purification; Bacterial Typing Techniques; Base Composition; DNA, Bacterial - genetics; Fatty Acids - chemistry; Indian Ocean; Nucleic Acid Hybridization; Phosphatidylethanolamines - chemistry; Phosphatidylglycerols - chemistry; Phylogeny; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; Sequence Analysis, DNA; Ubiquinone - chemistry; exopolysaccharide; Southwest Indian Ridge; deep sea sediment; Alteromonas
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.002245

A novel exopolysaccharide-producing strain, designated as 5.12
, was isolated from a sediment sample from the Southwest Indian Ridge, Indian Ocean. The strain was Gram-stain-negative, motile, strictly aerobic, and oxidase- and catalase-positive. It grew optimally at 35 °C, at pH 6.0 and in the presence of 3.5 % (w/v) NaCl. Its major isoprenoid quinone was ubiquinone-8 (Q-8) and summed feature 3 (comprising C16 : 1ω6c and/or C16 : 1ω7c), C16 : 0 and C18 : 1ω7c were the major cellular fatty acids. The DNA G+C content was 46.1 mol%. 16S rRNA gene sequence analysis suggested that strain 5.12
is a member of the genus Alteromonas. Strain 5.12
exhibited close 16S rRNA gene sequence similarity to Alteromonas lipolytica JW12
(96.1 %), Alteromonas hispanica F-32
(95.9 %), Alteromonas confluentis DSSK2-12
(95.9 %), Alteromonas litorea TF-22
(95.6 %) and Alteromonas mediterranea DE
(95.5 %). Strain 5.12
contained phosphatidylethanolamine and phosphatidylglycerol as the major polar lipids. Owing to significant differences in the 16S rRNA gene sequences, as well as the phenotypic and chemotaxonomic characteristics, the novel isolate described here merits classification as a representative of a novel species of the genus Alteromonas, for which the name Alteromonas pelagimontana sp. nov. is proposed. The type strain of this species is 5.12
(LMG 29661
= MCC 3250
).