Arthrobacter echini sp. nov., isolated from the gut of a purple sea urchin, Heliocidaris crassispina

• Published in: International journal of systematic and evolutionary microbiology Vol. 66; no. 4; pp. 1887 - 1893
• Main Authors: Lee, June-Young, Hyun, Dong-Wook, Soo Kim, Pil, Sik Kim, Hyun, Shin, Na-Ri, Yun, Ji-Hyun, Jung, Mi-Ja, Kim, Min-Soo, Woong Whon, Tae, Bae, Jin-Woo
• Format: Journal Article
• Language: English
• Published: England 01-04-2016
• Subjects: Animals; Anthocidaris - microbiology; Arthrobacter - classification; Arthrobacter - genetics; Arthrobacter - isolation & purification; Bacterial Typing Techniques; Base Composition; DNA, Bacterial - genetics; Fatty Acids - chemistry; Gastrointestinal Tract - microbiology; Glycolipids - chemistry; Molecular Sequence Data; Nucleic Acid Hybridization; Peptidoglycan - chemistry; Phospholipids - chemistry; Phylogeny; Republic of Korea; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Vitamin K 2 - chemistry; Republic of Korea
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.000965

A novel strain, designated AM23T, was isolated from the gut of a purple sea urchin Heliocidaris crassispina collected from the coastal waters of the Korean island Dokdo. 16S rRNA gene sequence analysis showed that strain AM23T belonged to the genus Arthrobacter in the family Micrococcaceae and shared highest sequence similarity with Arthrobacter agilis DSM 20550T (98.77%). Strain AM23T was catalase-positive, oxidase-negative and grew optimally at 20 °C, in the presence of 1% (w/v) NaCl and at pH 7. The isolate was a Gram-stain-positive, non-motile, strictly aerobic and coccus-shaped bacterium. The major cellular fatty acids were anteiso-C15:0 and iso-C15:0. The polar lipids of strain AM23T were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, one unidentified glycolipid and four unidentified lipids. The components of the cell-wall peptidoglycan were lysine, glutamic acid and alanine and the predominant cell-wall sugars were galactose, mannose, rhamnose and ribose. The major respiratory quinone was identified as menaquinone MK-9(H2). The genomic DNA G+C content was 67.3 mol% and the DNA-DNA hybridization values showed the strain shared less than 29% genomic relatedness with A. agilis DSM 20550T. The results of the phylogenetic, phenotypic and genotypic analysis indicate that strain AM23T represents a novel species in the genus Arthrobacter, for which the name Arthrobacter echini sp. nov. is proposed. The type strain is AM23T (=KACC 18260T=DSM 29493T).