Izhakiella capsodis gen. nov., sp. nov., in the family Enterobacteriaceae , isolated from the mirid bug Capsodes infuscatus

• Published in: International journal of systematic and evolutionary microbiology Vol. 66; no. 3; pp. 1364 - 1370
• Main Authors: Aizenberg-Gershtein, Yana, Laviad, Sivan, Samuni-Blank, Michal, Halpern, Malka
• Format: Journal Article
• Language: English
• Published: England 01-03-2016
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.000886

Gram-stain-negative, oxidase-negative, facultatively anaerobic, motile, rod-shaped, non-pigmented bacterial strains (N6PO6
, N8PO1 and N8PI1) were isolated from the mirid bug
captured on
plants. The 16S rRNA gene sequences of the strains shared 94.7-95.7 % similarity with species of the genus
and 95.6 % or less with species from other genera in the family
. A polyphasic approach that included determination of phenotypic properties and phylogenetic analysis based on 16S rRNA,
,
and
gene sequences supported the classification of strains N6PO6
, N8PO1 and N8PI1 as representing a novel species of a new genus in the family
. Strain N6PO6
, and the two reference strains of the novel species, grew at 1-37 °C, and in the presence of NaCl (up to 7.5 %, w/v) and sucrose (up to 60 %). Their major cellular fatty acids were C
, C
cyclo, C
ω7
, summed feature 2 (C
3-OH and/or iso-C
I) and summed feature 3 (C
ω7
and/or iso-C
2-OH). The DNA G+C content of strain N6PO6
was 49.9 mol%. On the basis of phenotypic properties and phylogenetic distinctiveness, the mirid bug isolates are classified as representing a novel species in a new genus
, in the family
, for which the name
gen. nov., sp. nov. is proposed. The type strain of
is N6PO6
( = LMG 28430
 = DSM 29293
).