GM-CSF secretion in primary cultures of normal and cancerous human renal cells

• Published in: Kidney international Vol. 50; no. 3; pp. 1044 - 1050
• Main Authors: Stephens, Nicole D., Barton, Sandra L., Smith, Anthony Y., Paul, Ralph W., Neidhart, James A., Griffith, Jeffrey K.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01-09-1996; Nature Publishing
• Subjects: Adenocarcinoma; Biological and medical sciences; Carcinoma, Renal Cell; Cell Count; Cell Line - cytology; Cell Line - metabolism; Cell Line - physiology; Granulocyte-Macrophage Colony-Stimulating Factor - genetics; Granulocyte-Macrophage Colony-Stimulating Factor - metabolism; Humans; Interleukin-1 - metabolism; Kidney - cytology; Kidneys; Medical sciences; Nephrology. Urinary tract diseases; Polymerase Chain Reaction; RNA, Messenger - metabolism; Tumor Cells, Cultured - cytology; Tumor Cells, Cultured - metabolism; Tumor Cells, Cultured - physiology; Tumors of the urinary system; Human; Urinary system disease; Carcinoma; Secretion; Cytokine; Renal disease; Malignant tumor; In vitro; Kidney; Polypeptide; Medium effect; Established cell line; Grawitz tumor; Granulocyte macrophage colony stimulating factor
• ISSN: 0085-2538; 1523-1755
• DOI: 10.1038/ki.1996.407

GM-CSF secretion in cultures of normal and cancerous human renal cells. Rates of secretion of granulocyte-macrophage colony stimulating factor (GM-CSF) were measured in 50 primary cell cultures derived from cancerous and normal human kidneys. Mean rates of GM-CSF secretion measured by TF-1 cell proliferation assay (N = 21) and by ELISA (N = 31) were 2.5 and 7.8 ng/106 cells/24 hr, respectively. There was no significant difference between the mean rates of GM-CSF secretion by cancerous and normal renal cells. GM-CSF was also secreted by primary renal cell cultures grown in serum-free medium and by renal cell lines. GM-CSF mRNA was detected by RT-PCR in cultured renal cells, but not in undissociated kidney tissue. Rates of GM-CSF secretion were reduced up to 99% under conditions where the cellular density or substratum more closely resembled the in vivo environment. Some cultured human renal carcinoma cells (RCC) secreted GM-CSF at levels that occasionally overlapped the levels produced by the GM-CSF gene-modified human RCC vaccine now in phase I trial. The data indicate that GM-CSF is not expressed in vivo, and that stable GM-CSF secretion is induced by the dissociation and culture of human renal cells.