Salivary and serum oxidative stress biomarkers and advanced glycation end products in periodontitis patients with or without diabetes: A cross‐sectional study

• Published in: Journal of periodontology (1970) Vol. 92; no. 9; pp. 1274 - 1285
• Main Authors: Altıngöz, Sema Merve, Kurgan, Şivge, Önder, Canan, Serdar, Muhittin A., Ünlütürk, Uğur, Uyanık, Metin, Başkal, Nilgün, Tatakis, Dimitris N., Günhan, Meral
• Format: Journal Article
• Language: English
• Published: United States 01-09-2021
• Subjects: 4‐hydroxy‐2‐nonenal; 8‐hydroxy‐2‐deoxyguanosine; Dentistry; diabetes mellitus; oxidative stress; periodontitis; type 2; diabetes mellitus; type 2; 4-hydroxy-2-nonenal; oxidative stress; 8-hydroxy-2-deoxyguanosine; periodontitis
• ISSN: 0022-3492; 1943-3670
• DOI: 10.1002/JPER.20-0406

Background
Non‐invasive methods for periodontitis diagnosis would be a clinically important tool. This cross‐sectional study aimed to investigate the association between oxidative stress, glycation, and inflammation markers and periodontal clinical parameters in periodontitis and periodontally healthy patients with type 2 diabetes and corresponding systemically healthy controls.
Methods
Sixty‐seven periodontally healthy (DM‐H, n = 32) and periodontitis (DM‐P, n = 35) patients with type 2 diabetes, and 54 systemically healthy periodontitis (H‐P, n = 26) and periodontally healthy (H‐H, n = 28) controls were included. Clinical periodontal parameters, body mass index, fasting glucose, hemoglobin A1c (HbA1c), along with saliva and serum 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG), malondialdehyde (MDA), 4‐hydroxy‐2‐nonenal (4‐HNE), advanced glycation end products (AGE), AGE receptor (RAGE) and high sensitivity C‐reactive protein (hsCRP) levels were recorded and analyzed.
Results
Salivary 8‐OHdG levels were significantly higher in periodontitis compared to periodontally healthy patients, regardless of systemic status (P < 0.001). Salivary MDA levels were significantly higher in all disease groups compared to H‐H group (P ≤ 0.004). Serum AGE levels were significantly higher in diabetic groups than systemically healthy groups (P < 0.001) and in H‐P compared to H‐H (P < 0.001). Bleeding on probing (BOP) and clinical attachment level (CAL) strongly correlated with salivary 8‐OHdG and serum hsCRP (P < 0.001). In systemically healthy patients, salivary 8‐OHdG was the most accurate marker to differentiate periodontitis from controls (AUC = 0.84). In diabetics salivary 4‐HNE and RAGE were the most accurate (AUC = 0.85 for both).
Conclusion
Salivary 8‐OHdG alone or in combination with 4‐HNE, AGE and RAGE for diabetics, and salivary 8‐OHdG alone or in combination with MDA and hsCRP for systemically healthy persons, could potentially serve as non‐invasive screening marker(s) of periodontitis.