The quantitative determination of several inhibitors of the angiotensin-converting enzyme by CE

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 25; no. 5-6; pp. 775 - 783
• Main Authors: HILLAERT, S, VAN DEN BOSSCHE, W
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Science 01-07-2001
• Subjects: Analysis; Angiotensin-Converting Enzyme Inhibitors - analysis; Biological and medical sciences; Electrophoresis, Capillary - methods; General pharmacology; Hydrogen-Ion Concentration; Medical sciences; Pharmacology. Drug treatments; Reference Standards; Reproducibility of Results; Tablets - chemistry; Validation; Separation method; Ultraviolet detector; Capillary electrophoresis; Dosage form; Tablet; ACE inhibitor; Optimization; Quantitative analysis
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/S0731-7085(01)00366-1

Capillary electrophoresis (CE) was applied to the study of several inhibitors of the angiotensin-converting enzyme. Separation of the compounds was performed by means of two phosphate buffers (each 100 mM) at pH 7.0 and 6.25, respectively [S. Hillaert, W. Van den Bossche, J. Chromatogr. A, 895 (2000) 33-42.]. Due to the highest selectivity of the first mentioned running buffer, the same system has been applied for the quantification of enalapril, lisinopril, quinapril, fosinopril, perindopril and benazepril in their corresponding pharmaceutical formulation. Especially, the possibility of simultaneous identification and quantification of the active ingredient in the finished product is very attractive. Excipients do not adversely affect the results. This paper deals with the validation of some parameters of the quantitative analysis: linearity, precision, accuracy and robustness.