Analysis of Post-Sample Collection EDTA Effects on Mean Platelet Volume Values in Relation to Overweight and Obese Patient Status

• Published in: Acta haematologica Vol. 142; no. 3; p. 149
• Main Authors: Pinto, Renata V L, Rodrigues, Genilson, Simões, Rafael L, Porto, Luís Cristóvão
• Format: Journal Article
• Language: English
• Published: Switzerland 01-09-2019
• Subjects: Adult; Aged; Blood Platelets - metabolism; Edetic Acid - pharmacology; Female; Humans; Male; Mean Platelet Volume; Middle Aged; Obesity - blood; Specimen Handling; Ethylenediaminetetraacetic acid; Mean platelet volume; Obesity
• ISSN: 1421-9662
• DOI: 10.1159/000499101

Mean platelet volume (MPV) may be a useful biomarker for platelet activation in obese patients. However, storage duration and use of anticoagulant K3-ethylenediaminetetraacetic acid (EDTA) may interfere with these measurements.
The aim of this study was to measure the variability of MPV in obese patients following exposure to EDTA.
A total of 160 patients were divided into 3 groups according to body mass index (BMI; normal: <25 kg/m2; overweight: 25-30 kg/m2; obese: >30 kg/m2). Blood was collected in sterile tubes containing K3-EDTA. Blood cell counts were obtained using the CELL-DYN Ruby system immediately and 1, 2, and 3 h after collection.
MPV was found to be directly proportional to BMI. With the addition of EDTA, MPV was increased in the first hour after collection; MPV then decreased to levels that were lower than initial baseline measurements.
K3-EDTA use alters platelet volume. The time from collection to measurement should be standardized to reduce MPV value variance. MPV should be determined within 1 h of collection to avoid anticoagulant use-related interference.