Biosensors designed for environmental and food quality control based on screen-printed graphite electrodes with different configurations

• Published in: Analytical and bioanalytical chemistry Vol. 374; no. 1; pp. 25 - 32
• Main Authors: Avramescu, Alina, Andreescu, Silvana, Noguer, Thierry, Bala, Camelia, Andreescu, Daniel, Marty, Jean-Louis
• Format: Journal Article
• Language: English
• Published: Germany 01-09-2002
• Subjects: Acetaldehyde - analysis; Acetylcholinesterase - metabolism; Air - analysis; Aldehyde Dehydrogenase - metabolism; Animals; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Electrochemistry; Electrodes; Environmental Monitoring - instrumentation; Environmental Monitoring - methods; Environmental Pollution; Enzymes, Immobilized - metabolism; Food Analysis - instrumentation; Food Analysis - methods; Graphite - chemistry; L-Lactate Dehydrogenase - metabolism; Lactic Acid - analysis; Monophenol Monooxygenase - metabolism; Phenols - analysis; Quality Control; Rabbits; Wine - analysis
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-002-1312-0

Graphite electrodes fabricated by screen-printing have been used as amperometric detectors in biosensors based on NAD(+)-dependent dehydrogenases, tyrosinase, or genetically modified acetylcholinesterases. The mono-enzyme sensors have been optimized as disposable or reusable devices for detection of a variety of substrates important in the food industry ( D-lactic acid, L-lactic acid, acetaldehyde) or in environmental pollution control (phenols and dithiocarbamate, carbamate and organophosphorus pesticides). The sensors were prepared in four configurations differing in enzyme confinement, enzyme immobilization and location of the immobilization agent in the biosensor assembly. Tests on real samples have been performed with the biosensors; D-lactic acid and acetaldehyde have been detected in wine and phenols in air.