Synthesis of structured lipids enriched with medium-chain fatty acids via solvent-free acidolysis of microbial oil catalyzed by Rhizomucor miehei lipase

• Published in: Food science & technology Vol. 93; pp. 306 - 315
• Main Authors: Abed, Sherif M., Wei, Wei, Ali, Abdelmoneim H., Korma, Sameh A., Mousa, Ahmed H., Hassan, Hamada M., Jin, Qingzhe, Wang, Xingguo
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01-07-2018
• Subjects: Arachidonic acid; Caprylic acid; Lipase-catalyzed acidolysis; Microbial oil; Structured lipids; Microbial oil; Structured lipids; Caprylic acid; Lipase-catalyzed acidolysis; Arachidonic acid
• ISSN: 0023-6438; 1096-1127
• DOI: 10.1016/j.lwt.2018.03.057

In this work, lipase-catalyzed acidolysis of microbial oil (MO) from Mortierella alpina with caprylic acid (CA) was carried out in a solvent-free system to produce structured lipids (SLs) enriched with medium-chain fatty acids (MCFAs). Among four commercial immobilized lipases screened for their acidolysis efficiency, Lipozyme RM IM from Rhizomucor miehei displayed the highest ratio of CA incorporation. The influence of enzyme loading, substrate molar ratio, reaction temperature, and reaction time on the CA incorporation was investigated. The produced SLs contained 49.45% arachidonic acid at the sn-2 position and 29.7% CA at sn-1,3 positions. In addition, a total of 25 triacylglycerol (TAG) molecular species containing CA were generated following the enzymatic acidolysis by using ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry (UPLCQ-TOF-MS). The most abundant TAG species in the SLs were (8:0-20:4-8:0), (20:4-20:4-8:0), and (20:3-20:4-8:0) accounting for 11.23%, 9.42%, and 4.99%, respectively. Moreover, the SLs showed an obvious decrease in the melting and crystallization profiles in comparison with those of the native MO. The synthesized SLs containing MCFAs would have the combined advantages of providing quick energy and as a source of essential fatty acids.
•Novel SLs enriched with MCFAs were successfully synthesized from microbial oil.•The highest CA incorporation was achieved using Rhizomucor miehei lipase.•A total of 25 TAG molecular species containing CA were generated after acidolysis.•SLs exhibited an obvious decrease in melting and crystallization profiles.