Microfluidic reactor with immobilized enzyme-from construction to applications: A review

• Published in: Chinese journal of chemical engineering Vol. 30; no. 2; pp. 136 - 145
• Main Authors: Zhang, Han, Bai, Yunpeng, Zhu, Ning, Xu, Jianhe
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-02-2021; State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai 200237,China%College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing,211800,China
• Subjects: Bioanalysis; Biocatalysis; Bioconversion; Immobilization strategies; Microfluidic immobilized enzyme reactor; Immobilization strategies; Bioconversion; Biocatalysis; Bioanalysis; Microfluidic immobilized enzyme reactor
• ISSN: 1004-9541; 2210-321X
• DOI: 10.1016/j.cjche.2020.12.011

Microfluidic, as the systems for using microchannel (micron- or sub-micron scale) to process or manipulate microflow, is being widely applied in enzyme biotechnology and biocatalysis. Microfluidic immobilized enzyme reactor (MIER) is a tool with great value for the study of catalytic property and optimal reaction parameter in a flourishing and highly producing manner. In view of its advantages in efficiency, economy, and addressable recognition especially, MIER occupies an important position in the investigation of life science, includingmolecularbiology, bioanalysisandbiosensing, biocatalysis etc. Immobilization of enzymes can generally improve their stability, and uponmostoccasions, the immobilized enzyme is endowed with recyclability. In this review, the enzyme immobilization techniques applied in MIER will be discussed, followed by summarizing the novel developments in the field of MIER for biocatalysis, bioconversion and bioanalysis. The preponderances and deficiencies of the current state-of-the-art preparation ways of MIER are peculiarly discussed. Inaddition, the prospects of its future study are outlined.