Biocompatibility, antioxidant activity and collagen photoprotection properties of C60 fullerene adduct with L-methionine

• Published in: Nanomedicine Vol. 40; p. 102500
• Main Authors: Sharoyko, Vladimir V., Shemchuk, Olga S., Meshcheriakov, Anatolii A., Vasina, Lubov V., Iamalova, Nailia R., Luttsev, Michail D., Ivanova, Daria A., Petrov, Andrey V., Maystrenko, Dmitriy N., Molchanov, Oleg E., Semenov, Konstantin N.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01-02-2022
• Subjects: Biological activity; Collagen; Fullerenes; L-Methionine; Ultra-violet light; PBS; UV; DPPH; MTT; ADP; C60-Met; PBMC; Biological activity; HRTEM; PRP; TGA; L-Methionine; Ultra-violet light; HEK293; DNA; Collagen; HSA; Fullerenes
• ISSN: 1549-9634; 1549-9642
• DOI: 10.1016/j.nano.2021.102500

Functionalization of the fullerene core with amino acids has become a new and promising direction in the field of nanochemistry. The biologic activity of water-soluble fullerene derivatives is based on such properties as lipophilicity, electron deficiency and photosensitivity. The complex of above-mentioned properties can be used to develop protection of biomolecules (in particular, proteins) from external physical and chemical influences. Thus, development and up-scaling of synthesis procedures, as well as investigation of the biological properties of these derivatives, are extremely important. This paper presents new data on the biocompatibility studies of C60 fullerene adduct with L-methionine (C60[C5H11NO2S]3; C60-Met). Antiradical activity, binding to human serum albumin (HSA), collagen and deoxyribonucleic acid (DNA), hemocompatibility, photodynamic properties, genotoxicity and cytotoxicity were studied. In addition, it was found that C60-Met increases the photostability of the collagen molecule, and this effect is dose-dependent.
The article presents novel data on hemocompatibility, binding to HSA and DNA, and cyto- and genotoxicity of the C60-Met adduct. Analysis of obtained data reveals that C60-Met possesses good biocompatibility. In addition, C60-Met exhibits antioxidant activity in experiments on interaction with DPPH and sodium nitroprusside, as well as in experiments on the Radachlorin photodegradation and on the photoinduced hemolysis. The indicated antioxidant properties of C60-Met determined its photoprotective effect towards collagen. [Display omitted]