Effect of recombinant porcine somatotropin and monoclonal antibody directed to ovine somatotrophic hormone on nitrogen retention and immune parameters in pigs

• Published in: Journal of animal science Vol. 72; no. 11; pp. 2820 - 2827
• Main Authors: Hel, W. van der (Agricultural University, Wageningen, The Netherlands), Parmentier, H.K, Hole, N.J.K, James, S, Brandsma, H.A, Vlissingen, J.M. van, Nieuwland, M.G.B, Joling, P
• Format: Journal Article
• Language: English
• Published: United States Am Soc Animal Sci 01-11-1994; Oxford University Press
• Subjects: AGONISTAS; AGONISTE; Animal Husbandry; Animals; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - pharmacology; Antibody Formation - drug effects; ANTICORPS MONOCLONAL; ANTICUERPOS MONOCLONALES; CERDO; EXCRECION; EXCRETION; Feces - chemistry; GAIN DE POIDS; GANANCIA DE PESO; Growth Hormone - immunology; Growth Hormone - pharmacology; Hogs; Immune System - cytology; Immune System - drug effects; Immune System - physiology; Immunity (Disease); IMMUNOGLOBULINE; INDICE DE CRECIMIENTO; INMUNOGLOBULINA; Leukocyte Count - veterinary; Lymphocytes - cytology; Male; Neutrophils - cytology; Nitrogen - analysis; Nitrogen - metabolism; Nitrogen - urine; PORCIN; PROMOTEUR DE CROISSANCE ANIMALE; PROMOTORES DEL CRECIMIENTO ANIMAL; Random Allocation; Recombinant Proteins - pharmacology; RETENCION NITROGENADA; RETENTION AZOTEE; Sheep; SOMATOTROPINA; SOMATOTROPINE; Swine - immunology; Swine - metabolism; TAUX DE CROISSANCE; Veehouderij; Weight Gain - drug effects; Weight Gain - physiology; WIAS
• ISSN: 0021-8812; 1525-3163
• DOI: 10.2527/1994.72112820x

Single and combined effects of administration and withdrawal of recombinant porcine somatotropin (rpST) and an enhancing murine anti-ovine growth hormone monoclonal antibody (OA15) on nitrogen retention, and serological and immunological measurements in pigs were examined in a placebo-controlled experiment. Thirty-six barrows were allotted to one of four treatments: control, rpST, OA15, and OA15+rpST. The trial phase was four balance periods: a preperiod, two periods of treatment, and a postperiod. Weight- and nitrogen gain were higher for the rpST group by 13% (P .01) and 15% (P .001), for the OA15 group by 8% (P .05) and 9% (P .05), and for the OA15+rpST group by 25% (P .001) and 20% (P .001), respectively compared with the control group. During the postperiod, weight gain of the OA15- and the OA15+rpST group was 23% (P .001) and 22% (P .001) lower than that of the control group. Nitrogen gain during the postperiod was decreased by 19% (P .01) for the OA15 group compared with the control group. Single or combined administration of rpST or OA15 did not affect (P .10) cellular constituents in the blood of all groups during the periods of observation. Animals treated solely with rpST mounted a humoral immune response directed to rpST. This anti-rpST antibody response was, however, decreased ( P .01) in barrows treated with rpST and OA16 simultaneously. Also, a slight anti-rpST antibody response was noticed in barrows solely treated with OA15. Barrows treated with OA15 mounted an anti-mouse immunoglobulin antibody response that correlated with a decrease ( P 0.001) in gain in the second period of treatment. These titers were lower than those found in pigs treated with a non-relevant mouse immunoglobulin of similar isotype as OA15. The OA15 mimicked effects of rpST. This may have implications for application in pig industry