Polymorphism of growth hormone gene and its association with expected milk production traits in dairy bulls

• Published in: Journal of Applied Animal Research Vol. 27; no. 1; pp. 29 - 33
• Main Authors: Pal, Aruna, Bhattacharya, T.K, Sharma, Arjava, Indian Veterinary Research Insitute, Izatnagar (India). Animal Genetics Division);Chakravarty, A.K.(National Dairy Research Institute, Karnal (India). Dairy Cattle Breeding Division
• Format: Journal Article
• Language: English
• Published: Taylor & Francis Group 01-03-2005
• Subjects: Buffalo; CATTLE; DAIRY; expected predicted difference; GENES; GROWTH; growth hormone gene; MILK; milk production; PCR; PCR-RFLP; PRODUCTION; RFLP; WATER BUFFALO
• ISSN: 0971-2119; 0974-1844
• DOI: 10.1080/09712119.2005.9706532

To explore polymorphism in 4th exon, 4th intron and 5th exon of growth hormone gene in Karan Fries (KF) cattle and Murrah buffalo bulls and its association with expected milk producing ability, a 428 bp fragment was amplified and digested with Alu I restriction enzyme. Two alleles L and V corresponding to leucine and valine variant of growth hormone polypeptide and thus two genotypes LL and LV were identified in Karan Fries bulls, whereas only LL genotype was observed in Murrah bulls. Karan Fries bulls with LL genotype were found to have higher (P0.01) expected predicted difference with respect to milk production traits as compared to the LV heterozygotes.