Induction of Cell Killing, Micronuclei, and Mutation to 6-Thioguanine Resistance after Exposure to Low-Dose-Rate γ Rays and Tritiated Water in Cultured Mammalian Cells (L5178Y)

• Published in: Radiation research Vol. 91; no. 3; pp. 447 - 456
• Main Authors: Ueno, Akiko M., Furuno-Fukushi, Ikuko, Matsudaira, Hiromichi
• Format: Journal Article
• Language: English
• Published: United States Academic Press, Inc 01-09-1982
• Subjects: Animals; Cell culture techniques; Cell growth; Cell Line; Cell Nucleus - drug effects; Cell Nucleus - radiation effects; Cell Survival - drug effects; Cell Survival - radiation effects; Cultured cells; Dosage; Dose response relationship; Dose-Response Relationship, Radiation; Drug Resistance; Gamma Rays; Germ cells; Irradiation; Leukemia L5178; Lymphocytes; Mice; Mutation; Radiation dosage; Relative Biological Effectiveness; Thioguanine - pharmacology; Tritium
• ISSN: 0033-7587; 1938-5404
• DOI: 10.2307/3575884

Cell killing and induction of micronuclei and mutation to 6-thioguanine resistance were studied in growing cultured mammalian cells (L5178Y) after exposure for a fixed period to low-dose-rate γ rays and tritiated water (HTO) at comparable dose rates, from about 10 to 40 rad/hr. From the analysis of dose-response relationships, RBE values of HTO β rays relative to γ rays were calculated. These were 1.5 for cell killing, 2.0 for micronuclei, and 1.8 for mutation induction. Reported RBE values for HTO were reviewed.