Bio-guided isolation of androsta-1,4-dien-3,16-dione as a vasodilator active principle from the inflorescence of Ravenala madagascariensis Sonn. (Strelitziaceae)

• Published in: Natural product research Vol. 37; no. 5; pp. 809 - 818
• Main Authors: Rakotondramanana, Dina Andriamahavola, Razafindrakoto, Zoarilala Rinah, Donno, Dario, Tombozara, Nantenaina, Nalimanana, Nina Robertina, Andrianajara, Charles, Beccaro, Gabriele Loris, Ramanitrahasimbola, David, Nicoletti, Marcello
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis Ltd 01-03-2023
• Subjects: Animals; Aorta; Calcium chloride; Data analysis; Enzyme inhibitors; Ethanol; Ethanol - pharmacology; Glibenclamide; Inflorescence; Methylene blue; NG-Nitroarginine methyl ester; NMR; Nuclear magnetic resonance; Phenylephrine; Plant extracts; Plant Extracts - pharmacology; Plant tissues; Potassium chloride; Propranolol; Rats; Ravenala madagascariensis; Reagents; Strelitziaceae; Vascular tissue; Vasodilation; Vasodilator Agents - chemistry; androsta-1,4-dien-3,16-dione; antihypertensive virtue; Ravenala madagascariensis; vaso-relaxing activity
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2022.2089668

Androsta-1,4-dien-3,16-dione was isolated for the first time from the plant kingdom of the ethanolic extract of the
' inflorescence by the bio-guided method. Its structure was elucidated by NMR and MS spectroscopic data analysis. The vascular effects of ethanol extracts, fractions and androsta-1,4-dien-3,16-dione were assessed on the phenylephrine pre-contracted isolated rat aorta. The isolated compound exerted the most potent vaso-relaxing effect (EC
= 109.32 ± 15.82 µM) than the ethanol extract and fractions. The pharmacological mechanism of its vaso-relaxing action was analysed on isolated rat aorta using free-endothelial vascular tissue, specific contracting reagents (CaCl
and KCl), antagonist (propranolol), enzyme inhibitors (L-NAME, methylene blue) and channel blocker (glibenclamide). Its vaso-relaxing activity could be due, at least partly, to the non-specific inhibition of the calcic influx.