Plant regeneration from anther culture in Canadian cultivars of flax (Linum usitatissimum L.)

• Published in: Euphytica Vol. 102; no. 2; pp. 183 - 189
• Main Authors: Chen, Y.R. (Agriculture and Agri-Food Canada, Morden, Man. (Canada). Morden Research Centre), Kenaschuk, E.O, Procunier, J.D
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01-01-1998; Springer Nature B.V
• Subjects: Anther and ovule culture, utilization for haploid production; ANTHER CULTURE; anthers; Biological and medical sciences; Biotechnology; callus; Cell culture; Cultivars; CULTIVO DE ANTERAS; CULTURE D'ANTHERE; culture media; dark; Eukaryotic cell cultures; Flax; Fundamental and applied biological sciences. Psychology; genetic variation; genotype; HAPLOIDIA; HAPLOIDIE; HAPLOIDY; IN VITRO REGENERATION; LINUM USITATISSIMUM; Methods. Procedures. Technologies; orientation; Plant cells and fungal cells; Plant growth; REGENERACION IN VITRO; REGENERATION IN VITRO; regenerative ability; TEMPERATURA; TEMPERATURE; thidiazuron; tissue culture; VARIEDADES; VARIETE; VARIETIES; zeatin; Adenine derivatives; Culture medium; Anther; Temperature; Tissue culture; Environmental factor; Genotype; Micropropagation; Fiber crop; Cytokinin; Dose activity relation; Linum usitatissimum; Regeneration; Linaceae; Dicotyledones; Angiospermae; Plant growth substance; Spermatophyta; Application method; Phytohormone; Intraspecific comparison; Cultivar
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1023/A:1018321428210

The effect of culture of anthers at 35 °C for one to four days prior to culture at 25 °C in darkness, genotype, anther orientation on callus induction and shoot regeneration in anther culture of flax was investigated. The influence of type and concentrations of cytokinins in the regeneration medium on shoot regeneration was also investigated. The results suggested that culture of anthers at 35 °C prior to continuous culture at 25 °C in darkness did not significantly improve the percentage of anthers producing calli. However, culture of anthers at 35 °C for one day significantly increased the overall efficiency of regeneration compared to no culture temperature treatment. Genotypic effects were significant for the percentage of anthers producing calli and the overall efficiency of regeneration. Anther orientation showed no significant differences. The regeneration medium containing 4.5 μM zeatin had significantly higher percentage of calli forming shoots than the same basal medium containing 0.01 μM TDZ. The importance of these findings for flax breeding was discussed.[PUBLICATION ABSTRACT]