Failure to detect the presence of pluripotential haemopoietic stem cells in the mouse brain

• Published in: Journal of neuroimmunology Vol. 18; no. 3; p. 217
• Main Authors: Stĕdra, J, Lodin, Z, Rossmann, P, Hartman, J, Sterzl, J
• Format: Journal Article
• Language: English
• Published: Netherlands 01-06-1988
• Subjects: Animals; Bone Marrow Cells; Bone Marrow Transplantation; Brain - cytology; Colony-Forming Units Assay; Hematopoietic Stem Cells - cytology; Mice; Mice, Inbred BALB C; Mice, Inbred CBA; Neurons - transplantation; Radiation Injuries, Experimental - mortality; Radiation Injuries, Experimental - prevention & control; Spleen - cytology
• ISSN: 0165-5728
• DOI: 10.1016/0165-5728(88)90099-9

Single cell suspensions prepared from adult mouse brains were tested for the presence of pluripotential haemopoietic stem cells (colony-forming units, CFU) by transfer into an irradiated recipient and enumeration of the CFU in the recipient's spleen. In contrast to the findings of others (Bartlett, 1982), we did not detect CFU after injection of brain cell suspensions, although they were detectable after inoculation with bone marrow cells. The number of CFU in recipients after transfer of increasing numbers of brain cells was the same as that detected in the irradiated controls which had not received any transferred cells. Finally, cells from the brain, in contrast to bone marrow cells, were not able to protect recipient animals from the effects of lethal irradiation.