Details of T-DNA structural organization from a transgenic Petunia population exhibiting co-suppression

• Published in: Plant molecular biology Vol. 32; no. 6; pp. 1197 - 1203
• Main Authors: Cluster, P.D. (John Innes Centre, Colney Lane, Norwich (United Kingdom).), O'Dell, M, Metzlaff, M, Flavell, R.B
• Format: Journal Article
• Language: English
• Published: Netherlands 01-12-1996
• Subjects: ADN RECOMBINADO; ADN RECOMBINE; AGROBACTERIUM; Blotting, Southern; CALCONA; CHALCONE; CHALCONES; DNA Restriction Enzymes; DNA, Bacterial - analysis; DNA, Bacterial - chemistry; FENOTIPOS; FLEUR; FLORES; FLOWERS; GENE TRANSFER; GENETIC INHERITANCE; Genetic Linkage; Genetic Vectors; HEREDITE; HERENCIA GENETICA; NUCLEOTIDE SEQUENCE; PETUNIA; PHENOTYPE; PHENOTYPES; PIGMENTACION; PIGMENTATION; PLANTAS TRANSGENICAS; PLANTE TRANSGENIQUE; Plants, Genetically Modified - genetics; RECOMBINANT DNA; Repetitive Sequences, Nucleic Acid; SECUENCIA NUCLEOTIDICA; SEQUENCE NUCLEOTIDIQUE; Suppression, Genetic; TRANSFERENCIA DE GENES; TRANSFERT DE GENE; Transformation, Genetic; TRANSGENIC PLANTS
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/BF00041406

Analysis of Agrobacterium-transferred DNA (T-DNA) revealed strong correlations between transgene structures and floral pigmentation patterns from chalcone synthase (chs) co-suppression among 47 Petunia transformants. Presented here are the full details of T-DNA structural organization in that population. Sixteen transformants (34%) carried one T-DNA copy while 31 (66%) carried 106 complete and partial T-DNA elements in 54 linkage groups. Thirty linkage groups contained multiple T-DNA copies; 15 of these contained only contiguously repeated copies, 8 contained only dispersed copies and 7 contained both. Right-border inverted repeats were three times more frequent than left-border inverted or direct repeats. Large fragments of binary-vector sequences were linked to the T-DNA in seven plants.